Treated

Flower bud (%)

Leaf bud (%)

Twig (%)

Date

Temp.

JMy

CH

KH

JM

CH

KH

JM

CH

KH

October 11

−6˚C

23.5 ± 5.6a

26.7 ± 12.0a

20.4 ± 11.6b

22.1 ± 6.0a

21.6 ± 11.1a

29.6 ± 5.5a

83.4 ± 9.5a

44.4 ± 24.2a

66.7 ± 16.7a

−9˚C

7.0 ± 4.4a

47.8 ± 24.8a

81.0 ± 15.6a

26.0 ± 3.2a

42.8 ± 21.4a

53.8 ± 17.7a

89.0 ± 11.0a

88.9 ± 5.6a

88.9 ± 11.1a

November 11

−6˚C

8.3 ± 5.2a

26.3 ± 6.9a

3.3 ± 3.3b

0

11.6 ± 8.7a

6.1 ± 6.1b

0

0

0

−9˚C

9.3 ± 0.7a

18.3 ± 2.4a

27.4 ± 1.2a

10.0 ± 5.8a

17.7 ± 13.0a

44.4 ± 8.0a

61.0 ± 20.1a

50.0 ± 0b

72.2 ± 14.7a

−12˚C

12.7 ± 6.6a

24.2 ± 5.5a

39.4 ± 4.6a

13.3 ± 3.4a

42.4 ± 10.5a

33.3 ± 8.3ab

55.7 ± 5.7ab

100.0 ± 0a

100.0 ± 0a

December 11

−12˚C

17.4 ± 8.1b

30.0 ± 15.3b

26.7 ± 7.0b

0

31.3 ± 17.8b

36.0 ± 7.4b

11.1 ± 5.6b

44.3 ± 5.7b

44.3 ± 5.7b

−15˚C

25.0 ± 2.6b

10.0 ± 10.0b

75.0 ±25ab

0

30.0 ± 6.8b

24.3 ± 12.3b

22.2 ± 5.6b

50.0 ± 0b

55.7 ± 5.7b

−18˚C

100.0 ± 0a

100.0 ± 0a

100.0 ± 0a

100.0 ± 0a

93.3 ± 11.1a

100.0 ± 0a

100.0 ± 0a

100.0 ± 0a

100.0 ± 0a

−21˚C

100.0 ± 0a

100.0 ± 0a

100.0 ± 0a

100.0 ± 0a

93.3 ± 21.4a

100.0 ± 0a

100.0 ± 0a

100.0 ± 0a

100.0 ± 0a

January 12

−15˚C

4.1 ± 1.5b

12.5 ± 6.6b

0

0

0

0

0

0

0

−18˚C

4.9 ± 2.8b

1.8 ± 1.8b

5.8 ± 3.6b

6.4 ± 3.2b

0

0

0

0

0

−21˚C

95.8 ± 2.1a

100.0 ± 0a

100.0 ± 0a

100.0 ± 0a

100.0 ± 11.1a

100.0 ± 0a

44.4 ± 5.6b

72.2 ± 14.7a

66.7 ± 16.7a

−24˚C

100.0 ± 0a

100.0 ± 0a

100.0 ± 0a

100.0 ± 0a

100.0 ± 21.4a

100.0 ± 0a

100.0 ± 0a

100.0 ± 0a

72.2 ± 11.1a

February 12

−18˚C

1.8 ± 1.8c

0

5.8 ± 3.1b

0

0

10.3 ± 1.2c

0

0

0

−21˚C

58.7 ± 8.0b

49.1 ± 6.4b

33.9 ± 19.3b

50.8 ± 6.5b

49.1 ± 4.0b

43.6 ± 6.1b

33.3 ± 0b

72.2 ± 5.6b

55.6 ± 5.6b

−24˚C

100.0 ± 0a

100.0 ± 0a

100.0 ± 0a

100.0 ± 0a

100.0 ± 0a

100.0 ± 0a

100.0 ± 0a

100.0 ± 0a

100.0 ± 0a

March 12

−12˚C

0

0

0

0

0

0

0

0

0

−15˚C

17.3 ±10.2b

17.5 ± 1.3b

6.4 ± 3.9b

0

0

0

0

0

0

−18˚C

100.0 ± 0a

100.0 ± 0a

100.0 ± 0a

100.0 ± 0a

100.0 ± 0a

100.0 ± 0a

38.9 ± 5.6a

100.0 ± 0a

88.9 ± 11.1a

April 12

0

0

0

0

0

0

0

0

0

0

−3˚C

0

0

0

0

0

0

0

0

0

−6˚C

83.2 ± 1.6a

81.4 ± 6.2b

59.3 ± 20.6a

100.0 ± 0a

100.0 ± 0a

66.4 ± 2.2a

55.6 ± 5.6a

50.0 ± 9.6b

38.9 ± 5.6a

−9˚C

75.1 ± 7.4a

100.0 ± 0a

34.4 ± 8.7ab

95.2 ± 4.8a

100.0 ± 0a

39.9 ± 9.4b

77.8 ± 8.7a

88.9 ± 11.1a

38.9 ± 5.6a